diff --git a/doc/source/development/contributing.rst b/doc/source/development/contributing.rst
index 042d6926d84f5..d7b3e159f8ce7 100644
--- a/doc/source/development/contributing.rst
+++ b/doc/source/development/contributing.rst
@@ -946,7 +946,7 @@ extensions in `numpy.testing
 
 .. note::
 
-   The earliest supported pytest version is 4.0.2.
+   The earliest supported pytest version is 5.0.1.
 
 Writing tests
 ~~~~~~~~~~~~~
diff --git a/doc/source/getting_started/install.rst b/doc/source/getting_started/install.rst
index 663948fd46cf6..3ba3a2fd4be1b 100644
--- a/doc/source/getting_started/install.rst
+++ b/doc/source/getting_started/install.rst
@@ -177,7 +177,7 @@ pandas is equipped with an exhaustive set of unit tests, covering about 97% of
 the code base as of this writing. To run it on your machine to verify that
 everything is working (and that you have all of the dependencies, soft and hard,
 installed), make sure you have `pytest
-<http://docs.pytest.org/en/latest/>`__ >= 4.0.2 and `Hypothesis
+<http://docs.pytest.org/en/latest/>`__ >= 5.0.1 and `Hypothesis
 <https://hypothesis.readthedocs.io/>`__ >= 3.58, then run:
 
 ::
diff --git a/doc/source/whatsnew/v1.0.0.rst b/doc/source/whatsnew/v1.0.0.rst
index 54640ff576338..6cbf655db4a44 100644
--- a/doc/source/whatsnew/v1.0.0.rst
+++ b/doc/source/whatsnew/v1.0.0.rst
@@ -253,6 +253,7 @@ Other API changes
   See :ref:`units registration <whatsnew_1000.matplotlib_units>` for more.
 - :meth:`Series.dropna` has dropped its ``**kwargs`` argument in favor of a single ``how`` parameter.
   Supplying anything else than ``how`` to ``**kwargs`` raised a ``TypeError`` previously (:issue:`29388`)
+- When testing pandas, the new minimum required version of pytest is 5.0.1 (:issue:`29664`)
 -
 
 
diff --git a/pandas/compat/_optional.py b/pandas/compat/_optional.py
index fc66502710b0c..ce9079ce8864d 100644
--- a/pandas/compat/_optional.py
+++ b/pandas/compat/_optional.py
@@ -18,6 +18,7 @@
     "pandas_gbq": "0.8.0",
     "pyarrow": "0.9.0",
     "pytables": "3.4.2",
+    "pytest": "5.0.1",
     "s3fs": "0.3.0",
     "scipy": "0.19.0",
     "sqlalchemy": "1.1.4",
diff --git a/pandas/util/_tester.py b/pandas/util/_tester.py
index 0f5324c8d02ba..7822ecdeeb4d8 100644
--- a/pandas/util/_tester.py
+++ b/pandas/util/_tester.py
@@ -11,7 +11,7 @@ def test(extra_args=None):
     try:
         import pytest
     except ImportError:
-        raise ImportError("Need pytest>=4.0.2 to run tests")
+        raise ImportError("Need pytest>=5.0.1 to run tests")
     try:
         import hypothesis  # noqa
     except ImportError: