Seems like all the ```Wire``` objects use the macro ```AP3_Wire1_IOM``` when they should use ``` AP3_Wire1_IOM AP3_Wire2_IOM AP3_Wire3_IOM AP3_Wire4_IOM AP3_Wire5_IOM ``` See the [source code here ](https://github.com/sparkfun/Arduino_Apollo3/blob/5227c1cd46f7a7c8ef0c13f9dde1870ed454fb2f/libraries/Wire/src/Wire.cpp#L363-L389)